| Introduction to Quantitative Real-time PCR |
Polymerase Chain Reaction(PCR) is that a single copy of a particular sequence can be specifically amplified and detected. It's based on nucleic acid hybridization. It relies on the segment to be amplified, the primers, temperature for the DNA annealing and denaturation and existence of a temperature resistant enzyme etc.
Real time PCR is a reliable detection and measurement of products generated during each cycle of the PCR process which are directly proportionate to the amount of template prior to the start of the PCR process. It is necessary to have a method of detecting the accumulation of PCR products and an instrument in which to perform the thermocycling that is adapted to record the results in each PCR cycle in real time.
There are flood of reports recently about quantitative real-time PCR. This testifies the transformation of this technology from an experimental tool to the scientific mainstream. Many of the applications of quantitative real-time PCR include measuring mRNA expression levels, DNA copy number, transgene copy number and expression analysis, allelic discrimination etc. We are investigating the numerical and statistical tools for the analysis of quantitative real-time PCR data.
This page prepared by Jing Yi
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